FAQ: When should Taq DNA Polymerase be used in a primer extension reaction or for PCR?

It is an excellent polymerase choice for routine primer extensions throughout a wide range of template sources. When cost per reaction and yield are priorities, Taq DNA Polymerase is the industry standard. It is limited by its relatively low thermostability (half-life of 45 minutes at 94°C) and lack of a proofreading exonuclease domain. These characteristics make it a poor choice for work on long (>5 kb) extensions, on templates with a high degree of secondary structure, or in applications that strive to minimize error incorporation.