Home FAQs How are abasic sites repaired by the PreCR Reaction Mix? Are new nucleotides put in and ligated? Is the existing nucleotide repaired?

FAQ: How are abasic sites repaired by the PreCR Reaction Mix? Are new nucleotides put in and ligated? Is the existing nucleotide repaired?

The DNA repair enzymes recognize the abasic site and cut the DNA backbone. The polymerase replaces the missing base with the correct base and the ligase ligates the DNA.