FAQ: How does the level of star activity of NheI-HF compare to NheI?

The Fidelity Index which measures the minimum units to produce star activity was determined using the following conditions; 1X NEBuffer, 5% glycerol, substrate pXba (1µg/50 µl), incubated for 1 hour at 37˚C . The minimum number of units of NheI-HF required to produce star activity in the recommended rCutSmart buffer™  is (≥32,000 units). The minimum number of units of NheI required to produce star activity in the recommended NEBuffer r3.1 is (120 units).The minimum number of units of NheI required to produce star activity in rCusmart Buffer is (32 units).

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NheI-HF®