neb.sg will be undergoing maintenance beginning Saturday, October 1st, at 5am SGT and will last through Monday, October 3nd, at 3am SGT. Site search will not be available during this time. We apologize in advance for any inconvenience this may cause.
For PNGaseF and Protein Deglycosylation Mix we suggest Fetuin (NEB #P6042, supplied with the Protein Deglycosylation Mix). For PNGaseF, Endo H, and Endo Hf, we suggest RNase B (NEB #P7817). For Endo D we suggest Phospholipase A from bee venom. For Endo S we suggest mouse monoclonal IgG (NEB #E8032). For O-glycosidase we suggest p-Nitrophenyl galacto-N-bioside (Galβ1-3GalNAcα1pNP, or Core1-pNP). Alternatively, fetuin can be digested with neuraminidase, and neuraminidase plus O-glycosidase. The changes in molecular weight after glycan removal can be observed by SDS-PAGE.