Home FAQs Can I use Total RNA to make small RNA libraries or do I have to isolate or enrich the sample for small RNA?

FAQ: Can I use Total RNA to make small RNA libraries or do I have to isolate or enrich the sample for small RNA?

Small RNA libraries can be done using Total RNA. It is not necessary to enrich the sample for small RNA if small RNA species are in a concentration higher than 0.5%. Total RNA with a RIN (RNA Integrity Number) higher than 7 is recommended.