New England Biolabs is working diligently to ensure we keep our employees and their families safe, while maintaining our business continuity. Despite a "Stay At Home" advisory being put in place in Massachusetts, USA, we are deemed an essential business, and our manufacturing and distribution teams continue to be fully operational. We are continuing to work with our suppliers and distribution partners to ensure uninterrupted access to our products and technical support.
HomeFAQsCan Bst 2.0 DNA Polymerase be used to fill in 3' overhangs?
FAQ: Can Bst 2.0 DNA Polymerase be used to fill in 3' overhangs?
No, DNA polymerases cannot fill in 3' overhangs. To create blunt ends 3' overhangs must be removed. DNA Polymerase I, Large (Klenow) Fragment (NEB# M0210), T4 DNA Polymerase (NEB# M0203) and Mung Bean Nuclease (NEB# M0250) are the best choices to remove 3' overhangs.
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