New England Biolabs is working diligently to ensure we keep our employees and their families safe, while maintaining our business continuity. Despite a "Stay At Home" advisory being put in place in Massachusetts, USA, we are deemed an essential business, and our manufacturing and distribution teams continue to be fully operational. We are continuing to work with our suppliers and distribution partners to ensure uninterrupted access to our products and technical support.
Unfortunately we don't know exactly what ends McrBC leaves. There may even be a mixture of types of ends produced. If McrBC digestion products are to be ligated, we suggest treating the McrBC-digested DNA with Klenow Fragment (3´→5´ exo-) (NEB #M0212) or T4 DNA polymerase (NEB #M0203). This enzyme will remove 3' overhangs and fill-in 5' overhangs, while leaving blunt ends alone. This should leave you with only blunt ends, which could then be ligated.
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