FAQ: Are there differences in ligation efficiency for T4 RNA Ligase 2, truncated KQ, T4 RNA Ligase 2, truncated 227Q, and T4 RNA Ligase 2, truncated KQ?

In NEB tests, ligations using T4 RNA Ligase 2, truncated T4 RNA Ligase 2, truncated K227Q, and T4 RNA Ligase 2, truncated KQ on identical substrates show that ligation efficiency reaches the same endpoints. While, T4 Ligase 2, truncated KQ and T4 RNA Ligase 2, truncated require similar incubation times, T4 RNA Ligase 2, truncated K227Q may require longer incubation times to reach the reaction endpoint.