HomeFAQsWhat volume of DNA can be added into chemically competent cells?
FAQ: What volume of DNA can be added into chemically competent cells?
The volume of DNA to be added into competent cells does affect transformation efficiency. 1-5 µl of DNA (plasmid or ligation product) is recommended for 50 µl of competent cells. When transforming a Gibson Assembly product, add 1-2 µl of the reaction to 50 µl of competent cells.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.