FAQ: I would like to use SplintR Ligase in a workflow, but not use the provided reaction buffer. Can you tell me what reaction buffer components are needed?

We recommend a reaction with 20-50 mM buffering agent at a pH between 7.5-8.0. Additionally, magnesium should be present at ≥ 5mM for maximal activity. Manganese is not required but we observe an increase in activity when this divalent cation is present above 5 mM. ATP is necessary, but the enzyme is active over a broad range of concentrations (10 nM-1 mM).