Home FAQs How is NEBNext Methylated Adaptor (NEB #E7535) different from NEBNext adaptor for Illumina (NEB #E7335 & #E7350)?

FAQ: How is NEBNext Methylated Adaptor (NEB #E7535) different from NEBNext adaptor for Illumina (NEB #E7335 & #E7350)?

The sequences of NEBNext Methylated Adaptor and NEBNext Adaptor for Illumina are identical except that all cytosines in NEBNext adaptor are replaced with methylated cytosines. They both can be used to prepare libraries for Illumina sequencing, however, only the NEBNext Methylated Adaptor can be used to prepare bisulfite-converted libraries for Illumina sequencing.