FAQ: What are the advantages of using NEBNext® Q5 Hot Start HiFi PCR Master Mix?

NEBNext Q5 Hot Start HiFi PCR Master Mix provides a unique combination of ultra-high fidelity and robust amplification of NGS libraries, regardless of GC content. It gives even higher PCR yields than its predecessor, NEBNext High-Fidelity 2X PCR Master Mix. In addition, due to its hot start nature, NEBNext Q5 Hot Start HiFi PCR Master Mix is ideal for workflows involving automation. It is compatible with a variety of carboxylated, tosylated and streptavidin beads that may be carried over or included in the PCR step of library construction protocols.