Home FAQs Can I see adaptor dimers as a measure of library quality?

FAQ: Can I see adaptor dimers as a measure of library quality?

Yes. While a melt curve is not required for the kit, and omitting it will reduce run times, data from the melt analysis can be used for library quality information. When adaptor dimers are present at >5% of the library sample, a single, sharp melt peak can be observed at 81.5 °C. In all other cases, a broad or multi-peaked melt curve will be produced, indicative of the range and diversity of the library being amplified.