| NEB Buffer | HiFi Taq DNA Ligase Activity (%) | Effect on Fidelity |
|---|---|---|
| Hifi Taq DNA Ligase Buffer |
100 | - |
| Taq DNA Ligase Buffer |
38 | ↓↓↓ |
| phi29 Reaction Buffer1 |
54 | ↓↓ |
| Q5® Reaction Buffer1 |
73 | ↓ |
| Standard Taq Reaction Buffer1 |
90 | ↓ |
| ThermoPol Buffer®1 |
215 | ↓↓ |
1Supplemented with 1 mM NAD.
2Additional supplementation of polymerase buffers with 5 mM Mg may increase HiFi Taq DNA Ligase activity, but further reduce fidelity.
Help us celebrate our 50th anniversary! We have hidden 1,000 golden butterflies and are waiting for you to find them. They can be anywhere that you find NEB! Beginning April 15th, be sure to visit our website and tables at tradeshows and events you are attending. Visit our social media channels frequently for tips on where we have hidden the butterflies – and once you find one, either click or scan the code to be eligible for a 50th anniversary prize pack, as well as a grand prize trip to NEB headquarters in Ipswich, MA!.
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