FAQ: How much HiFi Taq DNA Ligase is required for a reaction?

A typical reaction uses 1 μL of HiFi Taq DNA Ligase in a 50 μL reaction volume. Please see the accompanying product information for details on reaction conditions including molar ratios or probes, target DNA, and enzyme.
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    Quick Tips - How do I calculate how much DNA to add to a ligation reaction?

    This video provides tips for calculating the ideal insert:vector molar ratio before performing a ligation reaction.