Home FAQs Should I add additional ATP with Exonuclease V if I am scaling up my reaction?

FAQ: Should I add additional ATP with Exonuclease V if I am scaling up my reaction?

Yes. Exonuclease V requires ATP to cleave ss/ds DNA. However, greater than 1 mM ATP can cause inhibition. If more than 1 μg of DNA needs to be removed, one should increase all components proportionally, and incubate the reaction at 37°C for 30 min.