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FAQ: Can α1-2,4,6 Fucosidase O cleave branched and complex substrates?

α1-2,4,6 Fucosidase O will cleave branched α1-6 fucose residues and has minimal activity on branched α1-4 fucose residues. The enzyme will not cleave branched α1-2 fucose residues.  Longer incubation times (4 hours to overnight) may be needed for digestion of complex oligosaccharide substrates with multiple branches.