Home FAQs Can the Nucleoside Digestion Mix be incubated with substrate for >1 hour?

FAQ: Can the Nucleoside Digestion Mix be incubated with substrate for >1 hour?

Yes, to ensure complete digestion, the Nucleoside Digestion Mix can be incubated with sample for longer time periods. No signal deterioration has been observed by incubating DNA or RNA samples with the Nucleoside Digestion Mix for longer durations (overnight) at 37°C. Samples containing a large number of modifications (particularly modifications at the 2´-position) may benefit from overnight incubation with the Nucleoside Digestion Mix in order to achieve complete digestion.