FAQ: What is the activity of the Nucleoside Digestion Mix in other buffers?

In order to achieve the highest activity possible with the Nucleoside Digestion Mix, we recommend using the supplied Nucleoside Digestion Mix Buffer, however, the Nucleoside Digestion Mix is active over a wide range of pH values (optimally between pH 4.0–7.0) and is also salt tolerant (up to 250 mM). Additionally, since the Nucleoside Digestion Mix requires ZnCl₂, reaction buffer should be free of any chelating agents (i.e., EDTA) and supplemented with 1 mM ZnCl₂ in order to optimize activity in a buffer other than the supplied Nucleoside Digestion Mix Reaction Buffer.

While the Nucleoside Digestion Mix is active in NEBuffer r1.1 supplemented with 1 mM ZnCl2, the Nucleoside Digestion Mix is not active in NEBuffer r2.1, r3.1 or rCutSmart Buffers.