Home FAQs Can I use T7 Endonuclease I genome editing (CRIPSR/Cas9,TALEN,ZFN) mismatch detection assays using unpurified PCR products?

FAQ: Can I use T7 Endonuclease I genome editing (CRIPSR/Cas9,TALEN,ZFN) mismatch detection assays using unpurified PCR products?

We recommend using 1-4 μL of the appropriate PCR reaction directly in a 50 ul T7 Endonuclease I reaction when the following PCR reagents are used:
-Q5® High-Fidelity DNA Polymerase with Q5® Reaction Buffer -OneTaq® DNA Polymerase. Under these conditions, purification of PCR products before digestion with T7 Endonuclease I is optional.  For purification of PCR products we recommend the Monarch PCR & DNA Cleanup kit.