Home FAQs Does the DNA need to be purified after a restriction digest and prior to the dephosphorylation step?

FAQ: Does the DNA need to be purified after a restriction digest and prior to the dephosphorylation step?

If the enzymes used in the restriction digest can be heat inactivated, then a purification step is not needed. If the enzymes used cannot be heat inactivated, then a clean-up step between the restriction digest and the dephosphorylation step is recommended.