FAQ: How do I choose between LunaScript® RT SuperMix Kit and ProtoScript first strand cDNA synthesis kits?

The first strand cDNA synthesis kits contain different reverse transcriptases with the major differences being their primary applications, format, and reaction temperature. Here is a summary of our three first strand cDNA synthesis kits.

Product
LunaScript RT SuperMix Kit (E3010)
ProtoScript II First Strand cDNA Synthesis Kit (E6560)
ProtoScript First Strand cDNA Synthesis Kit (E6300)
RT Luna reverse transcriptase ProtoScript II reverse transcriptase M-MuLV reverse transcriptase
Format Supermix Reaction mix, enzyme mix, primer Reaction mix, enzyme mix, primer
Primary Application Two-step RT-qPCR Endpoint RT-PCR up to 10kb Endpoint RT-PCR up to 5kb
Secondary Application Endpoint RT-PCR up to 3kb Two-step RT-qPCR Two-step RT-qPCR
Reaction Temperature Up to 65°C (55°C optimal) Up to 48°C (42°C optimal) Up to 45°C (42°C optimal)
RNA Input 1μg to 1pg 1μg to 1pg 1μg to 1pg
Incubation 25°C 2 min, 55°C 10 min, 95°C 1 min 25°C 5 min, 42°C 60 min, 85°C 5 min 25°C 5 min, 42°C 60 min, 85°C 5 min
Additional Features Blue tracking dye, fast 13 min protocol