FAQ: Is TEV Protease compatible with different reaction buffers?

An optimal reaction buffer is the supplied 10X TEV Protease Reaction Buffer (0.5M Tris-HCl pH 7.5, 5mM EDTA, 10mM DTT). However, the enzyme is also active in HEPES, potassium phosphate and sodium acetate buffers between pH values of 6 and 9.