FAQ: Are the NEBNext® Multiplex Oligos for Enzymatic Methyl-seq the same as NEBNext® Multiplex Oligos for Illumina®?

No. For the 96 reaction size, the i5 and i7 unique dual indices in 96-well plate format are the same between the two products for individual wells. However, the volume of NEBNext® Multiplex Oligos for Enzymatic Methyl-seq (Unique Dual Index Primer Pairs) has been optimized for the NEBNext Enzymatic Methyl-seq Kit (NEB #7120). The 24 reaction NEBNext® Multiplex Oligos for Enzymatic Methyl-seq (Unique Dual Index Primer Pairs) are unique. The adaptors supplied in each product are different, and in the NEBNext® Multiplex Oligos for Enzymatic Methyl-seq (Unique Dual Index Primer Pairs) has also been modified for use in the EM-seq workflow.