FAQ: What is the optimal reaction buffer for Thermolabile Proteinase K?

Thermolabile Proteinase K is compatible with a wide range of buffers: Optimal pH: 7–10 Salt: 0–1 M NaCl Buffer: Tris-HCl, HEPES, Tricine, and Tris-Acetate. The table below shows relative activities in common buffers. 
1X Buffer  
NEB1.1 +
NEB2.1 +
NEB3.1 +
CutSmart +++
Q5 Buffer +++
Q5 HighGC +
Thermopol +
T4 DNA Ligase ++