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HomeFAQsCan one use SHuffle cells to secrete my protein of interest?
FAQ: Can one use SHuffle cells to secrete my protein of interest?
Although SHuffle cells have an intact secretion apparatus, periplasmic production of proteins using SHuffle cells is not recommended. Proteins that require disulfide bonds for their folding can be oxidized in the cytoplasm, prior to its secretion, blocking the secretion of the protein.