FAQ: Can I skip UMI sequencing if I don’t need the UMIs for my applications?

Yes. You can skip UMI sequencing and set i7 index read to 8 bases in this case.

The design of the NEBNext Unique Dual Index UMI Adaptors allows flexible index sequencing modes, and they are compatible with single index reads (with and without UMI) and dual index reads (with and without UMI).