FAQ: Is the NEBNext ARTIC SARS-CoV-2 Companion Kit (Oxford Nanopore Technologies) compatible with the ARTIC Network nCoV-2019 sequencing protocol v3 (LoCost) or the Oxford Nanopore Technologies Eco PCR Tiling protocol?

This kit is broadly compatible with these protocols. However, it is essential to follow the NEBNext ARTIC RT-PCR protocol in order to achieve optimal amplicon amplification with the balanced primer set. Note that purchase of additional Q5 Hot Start High-Fidelity 2x Master Mix (NEB #M0494) will be required if these protocols are followed.