Home FAQs Which NEBNext adaptors and primers are recommended for use with the ARTIC kits for Illumina?

FAQ: Which NEBNext adaptors and primers are recommended for use with the ARTIC kits for Illumina?

Instrument NextSeq 1000/2000, NovaSeq, any instrument with barcode hopping issues
Recommendation Any of the four NEBNext Multiplex Oligos for Illumina (Unique Dual Index Primer Pairs) Sets 1-4: E6440, E6442, E6444, E6446. 
<96 samples Purchase the S size
≥96 samples Recommendation depends on how many samples user plans on pooling for each sequencing run:
If pooling > 96 samples for a single run, users would need multiple UDI Sets. This is more likely with high throughput instruments e.g. NovaSeq.
If  ≥96 samples will not be pooled together for a single run, recommend L size of one kit.
Instrument MiSeq and NextSeq 500/550
Recommendation Any NEBNext Oligos product can be used. 
If there is any chance that the libraries will be sequenced on NextSeq or NovaSeq, Unique Dual Index sets are recommended.
<96 samples If only MiSeqs are available and <96 samples are pooled, the plated 96 single-index primer set (E6609) is the most user-friendly.
≥96 samples All NEBNext Oligos can be used, and users should decide on their preferred product format.
Instrument MiniSeq
Recommendation Any NEBNext Oligos product can be used. 
If there is any chance that the libraries will be sequenced on NextSeq or NovaSeq, Unique Dual Index sets are recommended.
<96 samples If only MiniSeqs are available and <96 samples are pooled, the plated 96 single-index primer set (E6609) is the most user-friendly.
≥96 samples All NEBNext Oligos can be used, and users should decide on their preferred product format.