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HomeFAQsDo I need to perform the qPCR step every time
FAQ: Do I need to perform the qPCR step every time?
It is best to perform the qPCR step every time a new RNA sample is used and/or a new type of library is prepared (e.g., B-cell receptor vs. T-cell receptor vs. both). When preparing a replicate library, the same PCR cycle number can be used as in the original prep.