HomeFAQsCan I use the NEBridge Ligase Master Mix reaction to transform electrocompetent cells?
FAQ: Can I use the NEBridge Ligase Master Mix reaction to transform electrocompetent cells?
Yes. Transformation efficiency of electrocompetent cells is generally 10 times higher than chemical competent cells. We have tested direct transformation of the NEBridge reactions using NEB 10-beta electrocompetent E.coli (NEB #C3020). While following the electroporation protocol provided with NEB 10-beta, and using 1 µL of the Golden Gate Assembly reaction with 25 µL of NEB 10-beta electrocompetent cells, we did not experience any problems, such as arcing.