FAQ: Can Faustovirus Capping Enzyme (NEB #M2081) use GTP analogs in a capping reaction?

3′-modified GTP analogs such as 3′-Biotin-GTP (NEB #N0760S) and 3′-desthiobiotin-GTP (NEB #N0761S) are only weakly incorporated by Faustovirus Capping Enzyme. Vaccinia Capping Enzyme (NEB #M2080) is our recommendation for this use case.