FAQ: In an effort to standardize reaction conditions, the recommended incubation temperature has changed for ApaI (NEB #R0114), BaeI (NEB #R0613), BsrDI (NEB #R0574) and SmaI (NEB #R0141). Which incubation temperature should I use?

You can safely incubate at either temperature. Please note that for SmaI, we suggest incubating at 25°C if you are doing extended digestions (>1 hour).