For cloning experiments choose from several high efficiency competent cell strains. These E. coli strains are T1 phage resistant and are endA deficient for high quality plasmid preparations. Additionally, all competent cells from NEB are free of animal products.
NEB offers chemically competent Kluyveromyces lactis cells and variants of this strain that have been tailored for specific protein expression needs. These cells are suitable for transformation with any of our linearized pKLAC series expression vectors.
NEB offers several strains with varying levels of expression control, each with T1 phage resistance and extremely high transformation efficiencies.
Use this protocol for maximum transformation efficiency.
Follow these tips to get superior results.
NEB has a long history in recombinant protein expression and has developed a wide array of solutions for proteins that are difficult to express.
In this video, see how transformation plating can be fast and easy using glass beads.