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Discontinued
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Protocols for Discontinued
- 2: In Vitro Transcription of Short Templates (50-300 nt) (E2030)
- 5 Minute Transformation Protocol (C3009)
- 5 Minute Transformation Protocol (C3016)
- Adaptor Ligation of End Repaired DNA (E6260)
- Adaptor or Vector Ligation of cDNA Library (E6114)
- Agencourt® Ampure® Bead (Beckman Coulter) Preparation (E6080)
- Agencourt® Ampure® Beads (Beckman Coulter) Preparation - NEBNext Quick DNA Sample Prep Master Mix Set for 454 End Repair and dA-Tailing (E6090)
- Agencourt Ampure Beads (Beckman Coulter) Preparation (E6116)
- Agencourt AMPure XP Bead Clean-up and Size Selection of End Repaired DNA (E6260)
- Cellular Labeling (S9126)
- Cellular Labeling (S9131)
- Cellular Labeling (S9137)
- Cellular Labeling (S9139)
- Cellular Labeling (S9142)
- Cellular Labeling (S9216)
- Cellular Labeling (S9218)
- Cloning of ACP-tag Fusions in pACP-tag(m)-2 (N9322)
- Cloning of MCP-tag Fusions in pMCP-tag(m) Vector (N9317)
- Cloning with USER Enzyme
- CLuc Activity Assay Protocol II (Injector-equipped luminometers) (E3314)
- CLuc Activity Assay Protocol I (Luminometers without injectors) (E3314)
- Combination of TransPass D1 or TransPass D2 & TransPass V (M2561)
- Comet Assay - Modified for Detection of Oxidized Bases Using the Repair Endonucleases Fpg, hOGG1 and Endonuclease III (Nth)
- PCR Protocol for Crimson Taq DNA Polymerase (M0324)
- PCR Protocol for Crimson Taq DNA Polymerase with (Mg-free) Buffer (M0325)
- Detection by Fluorescent Imager (P9311)
- Detection using the Phototope-Star Detection Kit
- Expression of ACP-ADRβ2 Fusions (N9321)
- Expression of ACP-tag Fusions (N9322)
- Expression of MCP Fusions (N9317)
- Expression of MCP-GPI (N9320)
- First Strand cDNA Synthesis (E6114)
- First Strand cDNA Synthesis
- First Strand cDNA Synthesis Protocols (E6550)
- High Efficiency Transformation Protocol (C3009)
- High Efficiency Transformation Protocol (C3016)
- Cellular Labeling (S9101)
- Instructions for Cellular Labeling (S9215)
- Labeling CLIP-tag Fusion Proteins with BC-substrates (S9222)
- Labeling CLIP-tag Purified Protein In Vitro (P9311)
- Labeling Mammalian Cell Lysates (S9146)
- Labeling Mammalian Cell Lysates (S9235)
- Labeling of Proteins in vitro (S9137)
- Labeling of Proteins in vitro (S9216)
- Labeling of Proteins in vitro (S9218)
- Labeling of Proteins in vitro (S9101)
- Labeling Proteins in vitro (S9131)
- Labeling proteins in vitro (S9126)
- Labeling Proteins in vitro (S9139)
- Labeling Proteins in vitro (S9142)
- Labeling Proteins in vitro (S9146)
- Labeling Purified Proteins in vitro (S9235)
- Protocol for Labeling SNAP-tag Fusion Proteins with BG-substrates (S9149)
- Ligation of 3´ and 5´ Adaptors (E6120)
- Loading a Sample (P7708)
- M13 Amplification
- miRNA Detection (E3312)
- mRNA Fragmentation Protocol (E6114)
- mRNA Fragmentation Protocol (E6116)
- NEBNext Adaptor Ligation (E6080)
- NEBNext Quick DNA Sample Prep Master Mix Set for 454 End Repair and dA-Tailing Adaptor Ligation (E6090)
- NEBNext Adaptor Ligation (E6116)
- NEBNext End Repair and dA-Tailing (E6116)
- NEBNext End Repair and dA-Tailing (E6080)
- NEBNext Quick DNA Sample Prep Master Mix Set for 454 End Repair and dA-Tailing (E6090)
- NEBNext End Repair Module Protocol (E6060)
- NEBNext End Repair Module Protocol (E6260)
- NEBNext DNA Sample Prep Master Mix Set for 454 Fill-in and ssDNA Isolation Module Protocol (E6070, E6071)
- NEBNext Quick Ligation Module Protocol (E6060)
- NEBNext Small Fragment Removal (E6080)
- NEBNext Quick DNA Sample Prep Master Mix Set for 454 End Repair and dA-Tailing Small Fragment Removal (E6090)
- NEBNext Small Fragment Removal (E6116)
- Nick Translation and Amplification of Adaptor Ligated DNA (E6260)
- PCR Amplification (E6120)
- Perform a Second Purification of the Amplified DNA (E6260)
- Preparing and Loading Protein Ladders (P7711)
- Protocol for a Typical miRNA Methylation Reaction (M0228)
- Protocol for Expression Using T7 Express Sampler (C3009)
- Protocol for Expression Using T7 Express Iq (C3016)
- Protocol II (Injector-equipped luminometers) (E3308)
- Protocol I (Luminometers without injectors) (E3308)
- Purification of Adaptor Ligated DNA (E6260)
- Purification of Amplified DNA (E6260)
- Reaction Protocols for Protein Deglycosylation Mix (P6039)
- Reconstitution of BioLux Cypridina Luciferase Substrate (E3314)
- Reverse Transcription (E6120)
- RNase Contamination Assay Kit (E3320)
- Second Strand cDNA Synthesis (E6114)
- ShortCut® siRNA Mix Suggested Protocol
- Suggested Protocol for Loading a Sample (P7702)
- Suggested Protocol for Loading a Sample (P7709)
- PCR Protocol for Taq DNA Polymerase with ThermoPol II (Mg-free) Buffer (M0321)
- Transfection Guidelines (E3314)
- Transformation Protocol (C1007)
- Transformation K. lactis YCT284 Competent Cells (C1002)
- TransPass COS/293: Transfection Protocol
- TransPass D1 Protocol 1: Transfection in the presence of serum
- TransPass D1 Protocol 2: Transfection in the absence of serum
- TransPass D2 Protocol 1: Transfection in the presence of serum
- TransPass D2 Protocol 2: Transfection in the absence of serum
- TransPass™ HUVEC Transfection Reagent: Transfection Protocol
- TransPass P Protein: Transfection Protocol
- TransPass R2: Plasmid DNA and siRNA Transfection Protocol
- TransPass R2: siRNA Transfection Protocol
- View the video "Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging" in the Journal of Visualized Experiments (JoVE)
- Western Transfer Protocol for Anti-MBP Antiserum
- NEBNext® RNase III RNA Fragmentation Module (E6146)
- Pmal Piii Vector Protocol
- Reaction Conditions for Chemical Coupling with CoA-SH (S9352S)
- Labeling of Proteins in vitro (S9215)
- Control Reactions with ProtoScript® M-MuLV Taq RT-PCR Kit
- Standard Digest Using RE-Mix®
- Library Preparation Using NEBNext® Multiplex Small RNA Library Prep Set for SOLiD™ (Set 1) (E7450)
- Cell Surface Labeling ACP-Surface Starter Kit
- Labeling of Proteins in vitro for ACP-Surface Starter Kit
- Expression of SNAPf Fusions (N9185)
- Expression of SNAPf Fusions in SNAPf-H2B (N9186)
- Removal of terminal N-acetylglucosamine from the biantennary N-linked sugars of IgG
- Amplification Protocol for PicoPLEX WGA Kit
- First Strand cDNA Synthesis with AMV LongAmp™ Taq RT-PCR Kit
- First Strand cDNA Synthesis with ProtoScript® M-MuLV Taq RT-PCR Kit
- PCR Amplification with AMV LongAmp™ Taq RT-PCR Kit
- PCR Amplification with ProtoScript® M-MuLV Taq RT-PCR Kit
- Pre-Amplification Protocol for PicoPLEX WGA Kit
- Sample Preparation Methods for PicoPLEX WGA Kit
- Double Digest Protocol using Two RE-Mix® Enzymes
- Labeling CLIP-tag Fusion Proteins with BC-substrates (S9237)
- Labeling SNAP-tag Fusion Proteins with BG-substrates (S9155)
- Protocol for Labeling ACP- or MCP-tag Fusion Proteins with CoA Substrates.
- Reaction Conditions for Chemical Coupling (S9155)
- Reaction Conditions for Chemical Coupling (S9237)
- LongAmp™ Taq 2X Master Mix Protocol (E6060)
- NEBNext End Repair cDNA Library (E6114)
- PCR Amplification of Adaptor Ligated cDNA Library (E6114)
- PCR Protocol for Crimson LongAmp™ Taq DNA Polymerase (M0326)
- Expression of CLIPf-NK1R (N9216)
- Expression of CLIPf-Cox8A (N9217)
- Expression of CLIPf-H2B (N9218)
- Typical Reaction Conditions (P0732)
- Reaction Conditions for Chemical Coupling (S9222)
- Reaction Conditions for Chemical Immobilization (S9149)
- Typical Reaction Conditions for α2-3 Neuraminidase (P0728)
- Typical Reaction Conditions for β1-4 Galactosidase (P0730)
- Library Preparation Using NEBNext® Small RNA Library Prep Set for SOLiD™ (E6160)
- M13 Titer Protocol
- Protocol for use with NEBNext mRNA Library Prep Master Mix Set for Illumina (E6110)
- Trypsin Digestion Protocol using NEB Trypsin-ultra™ and the FASP Kit
- Please see manual (NEB #E6000) for protocols.
- Please see manual (NEB #E6100) for protocols
- Double Digest Protocol using One RE-Mix and One Standard Restriction Enzyme
- Protocol for Digestion Prior to droplet digital PCR (ddPCR)
- Protocol for Direct Digestion of gDNA during droplet digital PCR (ddPCR)
Legal Information
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
