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Protocols for Discontinued
- M13 Titer Protocol
- Protocol for use with NEBNext mRNA Library Prep Master Mix Set for Illumina (E6110)
- Trypsin Digestion Protocol using NEB Trypsin-ultra™ and the FASP Kit
- Typical Reaction Conditions for β1-4 Galactosidase (P0730)
- Library Preparation Using NEBNext® Small RNA Library Prep Set for SOLiD™ (E6160)
- Please see manual (NEB #E6100) for protocols
- Please see manual (NEB #E6000) for protocols.
- Double Digest Protocol using One RE-Mix and One Standard Restriction Enzyme
- 2: In Vitro Transcription of Short Templates (50-300 nt) (E2030)
- Perform a Second Purification of the Amplified DNA (E6260)
- Preparing and Loading Protein Ladders (P7711)
- PCR Amplification (E6120)
- Protocol for Expression Using T7 Express Iq (C3016)
- Protocol for Expression Using T7 Express Sampler (C3009)
- Protocol for a Typical miRNA Methylation Reaction (M0228)
- miRNA Detection (E3312)
- Nick Translation and Amplification of Adaptor Ligated DNA (E6260)
- Loading a Sample (P7708)
- M13 Amplification
- Ligation of 3´ and 5´ Adaptors (E6120)
- Labeling Purified Proteins in vitro (S9235)
- Protocol for Labeling SNAP-tag Fusion Proteins with BG-substrates (S9149)
- Labeling Proteins in vitro (S9142)
- Labeling Proteins in vitro (S9146)
- Labeling proteins in vitro (S9126)
- mRNA Fragmentation Protocol (E6116)
- NEBNext Quick DNA Sample Prep Master Mix Set for 454 End Repair and dA-Tailing Small Fragment Removal (E6090)
- NEBNext Small Fragment Removal (E6116)
- NEBNext Small Fragment Removal (E6080)
- NEBNext Quick Ligation Module Protocol (E6060)
- NEBNext DNA Sample Prep Master Mix Set for 454 Fill-in and ssDNA Isolation Module Protocol (E6070, E6071)
- NEBNext End Repair Module Protocol (E6060)
- NEBNext End Repair Module Protocol (E6260)
- NEBNext End Repair and dA-Tailing (E6080)
- NEBNext End Repair and dA-Tailing (E6116)
- NEBNext Quick DNA Sample Prep Master Mix Set for 454 End Repair and dA-Tailing (E6090)
- NEBNext Adaptor Ligation (E6116)
- NEBNext Adaptor Ligation (E6080)
- NEBNext Quick DNA Sample Prep Master Mix Set for 454 End Repair and dA-Tailing Adaptor Ligation (E6090)
- Labeling Proteins in vitro (S9139)
- mRNA Fragmentation Protocol (E6114)
- TransPass D1 Protocol 1: Transfection in the presence of serum
- TransPass COS/293: Transfection Protocol
- Transformation K. lactis YCT284 Competent Cells (C1002)
- Transformation Protocol (C1007)
- TransPass D1 Protocol 2: Transfection in the absence of serum
- PCR Protocol for Taq DNA Polymerase with ThermoPol II (Mg-free) Buffer (M0321)
- Suggested Protocol for Loading a Sample (P7709)
- Suggested Protocol for Loading a Sample (P7702)
- Transfection Guidelines (E3314)
- TransPass D2 Protocol 1: Transfection in the presence of serum
- TransPass™ HUVEC Transfection Reagent: Transfection Protocol
- TransPass D2 Protocol 2: Transfection in the absence of serum
- Western Transfer Protocol for Anti-MBP Antiserum
- View the video "Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging" in the Journal of Visualized Experiments (JoVE)
- TransPass P Protein: Transfection Protocol
- Protocol I (Luminometers without injectors) (E3308)
- Reconstitution of BioLux Cypridina Luciferase Substrate (E3314)
- TransPass R2: siRNA Transfection Protocol
- TransPass R2: Plasmid DNA and siRNA Transfection Protocol
- Reaction Protocols for Protein Deglycosylation Mix (P6039)
- Purification of Amplified DNA (E6260)
- Purification of Adaptor Ligated DNA (E6260)
- Protocol II (Injector-equipped luminometers) (E3308)
- ShortCut® siRNA Mix Suggested Protocol
- Second Strand cDNA Synthesis (E6114)
- RNase Contamination Assay Kit (E3320)
- Cellular Labeling (S9218)
- Cellular Labeling (S9216)
- Cellular Labeling (S9142)
- Cellular Labeling (S9137)
- Cellular Labeling (S9131)
- Cellular Labeling (S9126)
- Cellular Labeling (S9139)
- Detection using the Phototope-Star Detection Kit
- Detection by Fluorescent Imager (P9311)
- PCR Protocol for Crimson Taq DNA Polymerase with (Mg-free) Buffer (M0325)
- PCR Protocol for Crimson Taq DNA Polymerase (M0324)
- Reverse Transcription (E6120)
- Comet Assay - Modified for Detection of Oxidized Bases Using the Repair Endonucleases Fpg, hOGG1 and Endonuclease III (Nth)
- Combination of TransPass D1 or TransPass D2 & TransPass V (M2561)
- CLuc Activity Assay Protocol I (Luminometers without injectors) (E3314)
- CLuc Activity Assay Protocol II (Injector-equipped luminometers) (E3314)
- Cloning with USER Enzyme
- Cloning of MCP-tag Fusions in pMCP-tag(m) Vector (N9317)
- Cloning of ACP-tag Fusions in pACP-tag(m)-2 (N9322)
- Typical Reaction Conditions for α2-3 Neuraminidase (P0728)
- NEBNext® RNase III RNA Fragmentation Module (E6146)
- LongAmp™ Taq 2X Master Mix Protocol (E6060)
- NEBNext End Repair cDNA Library (E6114)
- PCR Amplification of Adaptor Ligated cDNA Library (E6114)
- Cell Surface Labeling ACP-Surface Starter Kit
- Labeling of Proteins in vitro for ACP-Surface Starter Kit
- Removal of terminal N-acetylglucosamine from the biantennary N-linked sugars of IgG
- Expression of SNAPf Fusions (N9185)
- Expression of SNAPf Fusions in SNAPf-H2B (N9186)
- Amplification Protocol for PicoPLEX WGA Kit
- First Strand cDNA Synthesis with AMV LongAmp™ Taq RT-PCR Kit
- PCR Amplification with AMV LongAmp™ Taq RT-PCR Kit
- First Strand cDNA Synthesis with ProtoScript® M-MuLV Taq RT-PCR Kit
- PCR Amplification with ProtoScript® M-MuLV Taq RT-PCR Kit
- Pre-Amplification Protocol for PicoPLEX WGA Kit
- Sample Preparation Methods for PicoPLEX WGA Kit
- Double Digest Protocol using Two RE-Mix® Enzymes
- Labeling CLIP-tag Fusion Proteins with BC-substrates (S9237)
- Labeling SNAP-tag Fusion Proteins with BG-substrates (S9155)
- Protocol for Labeling ACP- or MCP-tag Fusion Proteins with CoA Substrates.
- Reaction Conditions for Chemical Coupling (S9155)
- Reaction Conditions for Chemical Coupling (S9237)
- Expression of CLIPf-NK1R (N9216)
- PCR Protocol for Crimson LongAmp™ Taq DNA Polymerase (M0326)
- Expression of CLIPf-Cox8A (N9217)
- Expression of CLIPf-H2B (N9218)
- Typical Reaction Conditions (P0732)
- Reaction Conditions for Chemical Coupling (S9222)
- Reaction Conditions for Chemical Immobilization (S9149)
- Labeling of Proteins in vitro (S9215)
- Control Reactions with ProtoScript® M-MuLV Taq RT-PCR Kit
- Reaction Conditions for Chemical Coupling with CoA-SH (S9352S)
- Pmal Piii Vector Protocol
- Standard Digest Using RE-Mix®
- Library Preparation Using NEBNext® Multiplex Small RNA Library Prep Set for SOLiD™ (Set 1) (E7450)
- 5 Minute Transformation Protocol (C3016)
- 5 Minute Transformation Protocol (C3009)
- Agencourt AMPure XP Bead Clean-up and Size Selection of End Repaired DNA (E6260)
- Agencourt Ampure Beads (Beckman Coulter) Preparation (E6116)
- Agencourt® Ampure® Beads (Beckman Coulter) Preparation - NEBNext Quick DNA Sample Prep Master Mix Set for 454 End Repair and dA-Tailing (E6090)
- Agencourt® Ampure® Bead (Beckman Coulter) Preparation (E6080)
- Adaptor or Vector Ligation of cDNA Library (E6114)
- Adaptor Ligation of End Repaired DNA (E6260)
- Labeling Proteins in vitro (S9131)
- Labeling Mammalian Cell Lysates (S9235)
- Labeling Mammalian Cell Lysates (S9146)
- Labeling CLIP-tag Purified Protein In Vitro (P9311)
- Labeling CLIP-tag Fusion Proteins with BC-substrates (S9222)
- Instructions for Cellular Labeling (S9215)
- Cellular Labeling (S9101)
- Labeling of Proteins in vitro (S9101)
- Labeling of Proteins in vitro (S9137)
- Labeling of Proteins in vitro (S9218)
- Labeling of Proteins in vitro (S9216)
- Expression of ACP-ADRβ2 Fusions (N9321)
- Expression of ACP-tag Fusions (N9322)
- Expression of MCP-GPI (N9320)
- High Efficiency Transformation Protocol (C3016)
- High Efficiency Transformation Protocol (C3009)
- First Strand cDNA Synthesis (E6114)
- First Strand cDNA Synthesis Protocols (E6550)
- First Strand cDNA Synthesis
- Expression of MCP Fusions (N9317)
- Protocol for Digestion Prior to droplet digital PCR (ddPCR)
- Protocol for Direct Digestion of gDNA during droplet digital PCR (ddPCR)
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While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
