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DNA Modifying Enzymes & Cloning Technologies
NEB’s enzymology expertise sets it apart from competitors, allowing us to produce enzymes for molecular biology that deliver; our highly pure enzymes, over 250 of which are recombinant, offer exceptional performance and value.
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DNA Modifying Enzymes & Cloning Technologies includes these subcategories:
Protocols for DNA Modifying Enzymes & Cloning Technologies
- Sample preparation using provided buffer (N0364)
- Optimizing Restriction Endonuclease Reactions
- Double Digest Protocol with Standard Restriction Enzymes
- NEBuilder HiFi DNA Assembly Reaction Protocol
- NEBuilder® HiFi DNA Assembly Electrocompetent Transformation Protocol
- NEBuilder® HiFi DNA Assembly Chemical Transformation Protocol
- HiFi Taq DNA Ligase (M0647) Protocol
- Protocol for a substrate with 5-hydroxymethyluridine such as bacteriophage SP8 genomic DNA (M0659)
Application Notes for DNA Modifying Enzymes & Cloning Technologies
- Choosing the Right Exonuclease
- Quick Tips - Can I make multiple mutations with the Q5 site-directed mutagenesis kit?
- DNA Ligase Selection Chart
- Properties of DNA and RNA Ligases
- Troubleshooting Guide for Cloning
- Troubleshooting Tips for Ligation Reactions
- Tips for Maximizing Ligation Efficiencies
- Traditional Cloning Quick Guide
- A novel enzyme isolated from a hot spring metagenomic DNA library defines a new CAZy family (GH154) of exosialidases with an inverting catalytic mechanism (2018)
- Choosing the Right Exonuclease (2019)
- Single-Strand Specific, Plasmid Borne DNA Methyltransferases M.BceJIII and M.EcoGIX Regulate Plasmid and Single-Stranded Phage Replication. (2017)
- miRNA Detection by Ligation and Amplification of Complementary DNA Oligos Using SPlintR® Ligase (2015)
Tools & Resources
Brochures
Webinars
Selection Tools
Troubleshooting Guides
Usage Guidelines
Posters
- Mauris, J.and Evans, T.C., Jr. 2010. A human PMS2 homologue from Aquifex aeolicus stimulates an ATP-dependent DNA helicase. J Biol.Chem. 285(15), PubMedID: 20129926, DOI:
Publications related to DNA Modifying Enzymes & Cloning Technologies
Legal Information
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
