MBD-Fc and MCIp were originally developed by Michael Rehli at the University of Regensburg to improve the sensitivity and specificity of conventional CpG binding techniques.
Each kit contains sufficient reagents for the enrichment of methylated DNA from up to 100 μg of fragmented input DNA. If starting with 5 ng to 10 μg of input DNA per experiment, the kit provides sufficient reagents for 25 reactions. Store at 4°C. For long-term storage > 6 months, MBD2-Fc protein should be stored at -20°C.
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|Bind/Wash Reaction Buffer (5X)||4||5 X|
|NaCl High-Salt Elution Buffer (2M)||4||500 mM|
|MBD2a-Fc Protein||-20||2 mg/ml|
|Fragmented HeLa DNA||4||100 µg/ml|
|LINE Primers for Methylated Controls||4||100 µM|
|RPL30 Primers for Non-methylated Controls||4||100 µM|
|MirA Primers for Input Control||4||100 µM|
|Protein A Magnetic Beads||4||250 µl|
Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at firstname.lastname@example.org or 1-800-632-5227 x 8.
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.