The EpiMark® 5-hmC and 5-mC Analysis Kit can be used to analyze and quantitate 5-methylcytosine and 5-hydroxymethylcytosine within a specific locus. Complete conversion of 5-hmC to glucosylated 5-hmC in DNA by T4 β-glucosyltransferase (T4-BGT).
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|NEBuffer™ 4||-20||10 X|
|T4 Phage β-glucosyltransferase (T4-BGT)||-20||10,000 units/ml|
|Unmodified Control DNA||-20||0.1 ng/µl|
|5-mC Control DNA||-20||0.1 ng/µl|
|5-hmC Control DNA||-20||0.1 ng/µl|
|Forward and Reverse Control Primer Mix||-20||10 µM each|
|Proteinase K, Molecular Biology Grade||-20||800 mg/ml|
|Uridine Diphosphate Glucose||-20||25 X|
Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at firstname.lastname@example.org or 1-800-632-5227 x 8.
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.