NEBNext® mRNA Library Prep Master Mix Set for 454™

  • Catalog # E6116 was discontinued on May 12, 2014 ;
  • Product Information

    The NEBNext mRNA Library Prep Master Mix Set for 454 contains enzymes and buffers that are ideally suited for sample preparation for next-generation sequencing, and for preparation of expression libraries. Each of these components must pass rigorous quality control standards and are Lot Controlled, both individually and as a set of reagents.

    Figure 1: Relative size distribution of eukaryotic mRNA fragments as seen using the Bioanalyzer 2100.
    Poly (A)+ mRNA (40 ng) purified from the Universal Human Reference Total RNA (Stratagene) was fragmented in 1X NEBNext Magnesium RNA Fragmentation Buffer for 1–5 minutes at 94°C. Fragmentation Reaction was stopped in 1X NEBNext Fragmentation Stop Solution. Samples were diluted 1:10 in Nuclease-Free Water and analyzed in the Bioanalyzer 2100.
    Functional Validation
    Each set of reagents is functionally validated together through construction and sequencing of an RNA library on the 454 platform.

    Lot Control

    The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page

    Reagents Supplied

    The following reagents are supplied with this product:

    Store at (°C) Concentration
    Quick T4 DNA Ligase -20
    NEBNext Sizing Buffer 1.2 X
    TE Buffer
    NEBNext End Prep Enzyme Mix
    NEBNext End Repair Reaction Buffer 10 X
    NEBNext RNA Fragmentation Buffer 10 X
    NEBNext RNA Fragmentation Stop Solution 10 X
    Linear Acrylamide -20 10 mg/ml
    Random Primers
    NEBNext First Strand Synthesis Reaction Buffer 5 X
    NEBNext Second Strand Synthesis Enzyme Mix
    NEBNext Second Strand Synthesis Reaction Buffer 10 X
    Nuclease-free Water -20
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