The NEBNext mRNA Library Prep Master Mix Set for 454 contains enzymes and buffers that are ideally suited for sample preparation for next-generation sequencing, and for preparation of expression libraries. Each of these components must pass rigorous quality control standards and are Lot Controlled, both individually and as a set of reagents.Functional Validation
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|Quick T4 DNA Ligase||-20|
|NEBNext Sizing Buffer||1.2 X|
|NEBNext End Prep Enzyme Mix|
|NEBNext End Repair Reaction Buffer||10 X|
|NEBNext RNA Fragmentation Buffer||10 X|
|NEBNext RNA Fragmentation Stop Solution||10 X|
|Linear Acrylamide||-20||10 mg/ml|
|NEBNext First Strand Synthesis Reaction Buffer||5 X|
|NEBNext Second Strand Synthesis Enzyme Mix|
|NEBNext Second Strand Synthesis Reaction Buffer||10 X|
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