Please note that for ChIP DNA library construction we now recommend the NEBNext® Ultra™ II DNA Library Prep Kit (NEB #E7645).
The NEBNext ChIP-seq Library Prep Kit for Illumina® utilizes the original standard library prep workflow, which is longer than the NEBNext Ultra II DNA Library Prep Kit workflow, has more components, requires more clean-up steps and higher input amounts.
The NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina contains reagents for preparation of libraries for next-generation sequencing on the Illumina platform from 10 ng of ChIP DNA, in a standard workflow. Please note that adaptors and primers are not included in the kit and are available separately.
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|NEBNext High-Fidelity 2X PCR Master Mix (SAMPLE)||2 X|
|NEBNext dA- Tailing Reaction Buffer||10 X|
|Klenow Fragment (3´→5´ exo-)|
|Quick T4 DNA Ligase||-20|
|NEBNext End Repair Enzyme Mix|
|NEBNext End Repair Reaction Buffer||10 X|
|NEBNext Quick Ligation Reaction Buffer||-20||5 X|
|NEBNext® Q5® Hot Start HiFi PCR Master Mix||-20|
Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at firstname.lastname@example.org or 1-800-632-5227 x 8.
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.