The NEBNext Fast DNA Library Prep Set for Ion Torrent is suitable when mechanical methods, such as Covaris® acoustic shearing, are preferred for fragmentation of input DNA. The components and protocol for this kit have been optimized for the volumes and concentrations of DNA sheared by mechanical methods, and the kit is compatible with input amounts from 10 ng - 1µg, with a workflow time of under 2 hours and ~12 min. hands-on time..
DNA Library Preparation Workflow for Ion Torrent Representative Results from Libraries Constructed Using NEBNext for Ion Torrent 0.5 µg of DNA from 3 different genomes with varying GC content were used to construct 200 bp and 400 bp libraries using the NEBNext Fast DNA Fragmentation and Library Prep Set for Ion Torrent, analyzed by the Agilent® Bioanalyzer®.Representative Ion Torrent Run Report A typical Ion Torrent run report for libraries made from E. coli (K12 MG1655 strain) genomic DNA using NEBNext Fast DNA Fragmentation & Library Prep Set for Ion Torrent. A. Alignment Summary. 72 Mb of data was generated, with an average genome coverage of 15.5 X, from approximately 0.5 Million 200 bp reads. B. Read length histogram from 200 bp run.
Lot Control
The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page
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