NEBNext® Poly(A) mRNA Magnetic Isolation Module
Ribosomal RNAs (rRNAs) are extremely abundant, constituting 80–90% of total RNA, and efficient removal of rRNA is critical to enable cost-effective sequencing of RNA samples. Oligo dT-based mRNA isolation is ideal for enrichment of mRNA and separation from rRNA when samples are eukaryotic and have intact poly(A) tails.
This product is compatible with total RNA inputs of 1-5µg. For input amounts >5µg we recommend the NEBNext High Input Poly(A) mRNA Isolation Module (NEB #E3370).
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Product Information
The NEBNext Poly(A) mRNA Magnetic Isolation Module is designed to isolate intact poly(A)+ RNA from 1-5µg of previously isolated total RNA. The technology is based on the coupling of Oligo d(T)25 to 1 μm paramagnetic beads which is then used as the solid support for the direct binding of poly(A)+ RNA. Thus, the procedure permits the manual processing of multiple samples and can be adapted for automated high-throughput applications. Additionally, magnetic separation technology permits elution of intact mRNA in small volumes eliminating the need for precipitating the poly(A)+ transcripts in the eluent. Intact poly(A)+ RNA which is fully representative of the mRNA population of the original sample can be obtained in less than one hour.
Note that for input amounts >5µg we recommend the NEBNext High Input Poly(A) mRNA Isolation Module (NEB #E3370).
- This product is related to the following categories:
- RNA Depletion & mRNA Enrichment,
- RNA Library Prep for Illumina,
- Next Generation Sequencing Library Preparation,
- Small RNA Library Preparation,
- Microbiome DNA Enrichment,
- Automation for NEBNext® NGS Library Prep,
- Library Preparation for Ion Torrent,
- Library Preparation for Oxford Nanopore Technologies,
- ​FFPE DNA Repair,
- NEBNext® ARTIC products for SARS-CoV-2 sequencing,
- DNA Fragmentation & RNA Fragmentation,
- Infectious Diseases & ARTIC Sequencing,
- NEBNext® Multiplex Oligos (Adaptors & Primers),
- NGS Library Quantitation,
- Methylome Analysis,
- DNA Library Prep for Illumina, Library Preparation for Illumina
- This product can be used in the following applications:
- RNA Modification,
- RNA Purification and Isolation,
- RNA-seq
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