NEBNext® Ultra™ II End Repair/dA-Tailing Module
This module is part of the Ultra™ II workflow, and is optimized for use with the NEBNext™ Ultra II Ligation Module (NEB #E7595), for Illumina®-compatible library construction.
This module is also compatible with some Oxford Nanopore MinION™workflows.
Kits that include reagents for every step in the Ultra II DNA library construction workflow are also available (NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645) and NEBNext Ultra II DNA Library Prep with Sample Purification Beads (NEB #E7103)).
The NEBNext Ultra II End Repair/dA-Tailing Module has been optimized to convert 500 pg-1 μg of fragmented DNA to repaired DNA having 5′ phosphorylated, 3′ dA-tailed ends. The module is optimized for use with the NEBNext Ultra II Ligation Module (NEB #E7595), and is part of the Ultra II DNA workflow which enables high yield preparation of high quality libraries from 500 pg to 1 µg of input DNA.
This module is compatible with Illumina workflows and with some Oxford Nanopore MinION™workflows.
For Illumina library construction, the NEBNext Ultra II End Repair/dA-Tailing Module is designed for use with the following:
• NEBNext Ultra II Ligation Module (NEB #E7595)
• NEBNext Ultra II Q5® Master Mix (NEB #M0544)
• NEBNext Oligos for Illumina (NEB #E7335, #E7500, #E7710, #E7730, #E6609, #E7600 #E7535, #E7350)
For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact firstname.lastname@example.org for further information.
The following reagents are supplied with this product:
Store at (°C) Concentration NEBNext Ultra II End Prep Enzyme Mix -20 NEBNext Ultra II End Prep Reaction Buffer -20
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