NEBExpress® MBP Fusion and Purification System

This product is a direct replacement for NEB #E8200, pMAL™ Protein Fusion and Purification System

The NEBExpress® MBP Fusion and Purification System takes advantage of the strong Ptac promoter and the translation initiation signals of maltose binding protein (MBP) to enhance solubility and expression levels of a desired protein in E. coli. The resulting product is an MBP fusion protein, which is then purified by affinity chromatography.

  • Reliable E. coli expression: substantial yields (up to 100 mg/L)
  • Fusion to MBP has been shown to enhance the solubility of proteins expressed in E. coli (1)
  • Two-step purification: amylose elution followed by TEV Protease cleavage and Ni resin isolation results in a highly pure tag-free target protein
  • Gentle elution with maltose; no detergents or harsh denaturants required
 
Catalog # Concentration Size
E8201S Not Applicable 1 set
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