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Genome Editing
- EnGen® Spy Cas9 NLS, EnGen Lba Cas12a, EnGen Sau Cas9: for genome engineering by direct introduction of active nuclease complexes
- EnGen® sgRNA Synthesis Kit: for rapid generation of microgram quantities of custom sgRNA
- EnGen® Mutation Detection Kit: for robust determination of targeting efficiency
- EnGen® Spy Cas9 Nickase: programmable site-specific DNA nicking for genome editing and in vitro applications
- EnGen Spy dCas9 (SNAP-tag): Programmable DNA binding activity compatible with broad variety of SNAP-tag conjugates
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Protocols for Genome Editing
- EnGen® sgRNA Synthesis Kit, S. pyogenes Protocol (E3322)
- In vitro digestion of DNA with Cas9 Nuclease, S. pyogenes (M0386)
- T7 Endonuclease I-based Mutation Detection with the EnGen® Mutation Detection Kit (NEB #E3321)
- Transfection of Cas9 RNP (ribonucleoprotein) into adherent cells using the Lipofectamine® RNAiMAX
- Determining Genome Targeting Efficiency using T7 Endonuclease I
- Using recombinant Cas9 nuclease to assess locus modification in genome editing experiments (#M0386)
- Genome Editing Brochure
- Kinetic Comparison of Cas9 Homologs Recognizing Diverse PAM Sequences (2018)
Tools & Resources
Brochures
Posters
Legal Information
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Videos
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Bacterial Adaptive Immunity with CRISPR/Cas9
CRISPR systems evolved as a means of bacterial adaptive immunity. Understanding their behavior in vivo is essential to harnessing their power in vitro.
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Science in 60 CRISPR/Cas9
Get a high level overview of CRISPR/Cas9 and how it is used in genome editing in our Science in 60 segment.
