T4 DNA Polymerase
cloned at NEB recombinant neb31 75 Heat

We are excited to announce that we are in the process of switching all reaction buffers to be BSA-free. Beginning April 2021, we will be gradually transitioning to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. All information on the website has been updated to reflect this change. Find more details at www.neb.com/BSA-free.

T4 DNA Polymerase catalyzes the synthesis of DNA in the 5´→ 3´ direction and requires the presence of template and primer. 

  • Gap filling (no strand displacement activity)
  • Removal of 3’ overhangs or fill-in of 5’ overhangs to form blunt ends
  • Lacks 5’ —> 3’ exonuclease activity
  • Probe labeling using replacement synthesis
  • Singe-strand deletion subcloning
Catalog # Concentration Size
M0203S 3,000 units/ml 150 units
M0203L 3,000 units/ml 750 units
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