DNA Polymerase I, Large (Klenow) Fragment
NEB2 cloned at NEB recombinant 25 75 Heat

DNA Polymerase I, Large (Klenow) fragment was originally derived as a proteolytic product of E.coli DNA polymerase that retains polymerase and 3’ —> 5’ exonuclease activity

  • Removal of 3’ overhangs or fill-in of 5’ overhangs to form blunt ends
  • Lacks 5’ —> 3’ exonuclease activity
  • Generates probes using random primers
  • Second strand cDNA synthesis
Catalog # Concentration Size
M0210S 5,000 units/ml 200 units
M0210L 5,000 units/ml 1,000 units
M0210M 50,000 units/ml 1,000 units
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