Vent^® (exo-) DNA Polymerase

Reagents Supplied

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0257S     -20       Vent® (exo-) DNA Polymerase M0257SVIAL -20 1 x 0.1 ml 2,000 units/ml   ThermoPol® Reaction Buffer B9004SVIAL -20 1 x 1.5 ml 10 X   Magnesium Sulfate (MgSO4) Solution B1003SVIAL -20 1 x 1.5 ml 100 mM
M0257L     -20       Vent® (exo-) DNA Polymerase M0257LVIAL -20 1 x 0.5 ml 2,000 units/ml   ThermoPol® Reaction Buffer B9004SVIAL -20 3 x 1.5 ml 10 X   Magnesium Sulfate (MgSO4) Solution B1003SVIAL -20 1 x 1.5 ml 100 mM

Application Features

• PCR
• Primer extension
• Thermal cycle sequencing
• High temperature dideoxy-sequencing

Companion Products

• Deoxynucleotide (dNTP) Solution Set
• Deoxynucleotide (dNTP) Solution Mix

Materials Sold Separately

• ThermoPol® Reaction Buffer Pack
• Magnesium Sulfate (MgSO₄) Solution

1. BSA is not provided with this enzyme since its presence is not necessary for most primer extension reactions. However, it is available as NEB #B9001. Acetylated BSA should not be used for primer extension reactions.
2. Diluent D is also available. This diluent is recommended for making dilutions of Vent (exo-) Polymerase.
3. Additional ThermoPol Reaction Buffer Packs for this product are also available. Each buffer pack contains 4 vials of 10X buffer (1.5 ml each) and 1 vial of 100 mM MgSO₄.

1. Kong, H.M., Kucera, R.B. and Jack, W.E. (1993). J. Biol. Chem.. 268, 1965-1975.
2. Mattila, P. et al. (1991). NAR. 19, 4967-4973.
3. Eckert, K.A. and Kunkel, T.A. et al. (1991). PCR Methods and Applications 1. 17-24.
4. Perler, F.B. et al. (1992). PNAS USA. 89, 5577-5581.
5. Belkin, S. and Jannasch, H.W. (1985). Arch Microbiol. 141, 181-186.

1. Guidelines for PCR Optimization for Vent DNA Polymerase
2. Protocol for a Routine Vent (exo-) PCR

• Guidelines for PCR Optimization with Thermophilic DNA Polymerases

• DNA Polymerase Selection Chart
• Thermophilic DNA Polymerases

1. Can Vent (exo-) DNA Polymerase be used in other buffers?
2. Are the DNA fragments produced by Vent (exo-) DNA Polymerase blunt-ended or do they have the single-base 3' overhang that Taq DNA Polymerase yields?
3. I can't get Vent (exo-) DNA Polymerase to work yet Taq DNA Polymerase works fine.
4. Which thermophilic DNA polymerase should I use?
5. What should I take into consideration when designing a set of PCR primers?
6. How can I facilitate the amplification of templates with hairpin-loop structures or high GC-content?

• PCR Troubleshooting Guide

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0257S_L_v1
• M0257S_L_v2

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0257S_L_v1_0171606
• M0257S_L_v1_0171612
• M0257S_L_v1_0171706
• M0257S_L_v1_0171712
• M0257S_v1_10011627
• M0257L_v1_10015889
• M0257S_v1_10029417
• M0257L_v1_10030652
• M0257S_v1_10034085
• M0257L_v1_10043809
• M0257S_v1_10055120
• M0257L_v1_10060276
• M0257S_v1_10062084
• M0257L_v2_10078993
• M0257S_v2_10082006
• M0257L_v2_10099433
• M0257S_v2_10107183
• M0257L_v2_10123093
• M0257S_v2_10127768
• M0257L_v2_10140868
• M0257S_v2_10146111
• M0257L_v2_10151440
• M0257S_v2_10159371
• M0257L_v2_10167680
• M0257S_v2_10172269

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Vent^® (exo-) DNA Polymerase
• ThermoPol^® Reaction Buffer
• Magnesium Sulfate (MgSO₄) Solution

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Licenses

Some applications in which this product can be used may be covered by patents issued and applicable in the United States and certain other countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license depending upon the particular application in which the product is used.

Trademarks

NEW ENGLAND BIOLABS®, VENT® and THERMOPOL® are registered trademarks of New England Biolabs, Inc.
DEEP VENT^® is a trademark of New England Biolabs, Inc.

TRITON® is a registered trademark of Union Carbide Corporation