T7 DNA Polymerase (unmodified)

Reagents Supplied

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0274S     -20       T7 DNA Polymerase (unmodified) M0274SVIAL -20 1 x 0.03 ml 10,000 units/ml   T7 DNA Polymerase Reaction Buffer B0274AVIAL -20 1 x 1.5 ml 10 X   Recombinant Albumin, Molecular Biology Grade B9200SVIAL -20 1 x 0.6 ml 20 mg/ml
M0274L     -20       T7 DNA Polymerase (unmodified) M0274LVIAL -20 1 x 0.15 ml 10,000 units/ml   T7 DNA Polymerase Reaction Buffer B0274AVIAL -20 1 x 1.5 ml 10 X   Recombinant Albumin, Molecular Biology Grade B9200SVIAL -20 1 x 0.6 ml 20 mg/ml

Application Features

• Second strand synthesis in site-directed mutagenesis protocols (8).

Companion Products

• Deoxynucleotide (dNTP) Solution Mix
• Deoxynucleotide (dNTP) Solution Set
• Monarch® Plasmid Miniprep Kit 
• Monarch® DNA Gel Extraction Kit
• Monarch® PCR & DNA Cleanup Kit (5 μg)

Materials Sold Separately

• Recombinant Albumin, Molecular Biology Grade
  
  
  
  
  

1. The high polymerization rate of the enzyme makes long incubations unnecessary.
2. T7 DNA Polymerase is not suitable for DNA sequencing.

1. Hori, K. et al. (1979). J. Biol. Chem.. 254, 11598-11604.
2. Engler, M.J. et al. (1983). J. Biol. Chem.. 258, 11165-11173.
3. Nordstrom, B. et al. (1981). J. Biol. Chem.. 256, 3112-3117.
4. Studier, F.W. et al. (1990). Goeddel, D.V.(Ed.), In Methods Enzymol.. 185, 60-89. San Diego: Academic Press.
5. Grippo, P. and Richardson, C.C. (1971). J. Biol. Chem. 246, 6867-6873.
6. Modrich, P. and Richardson, C.C. (1975). J. Biol. Chem. 250, 5515-5522.
7. Adler, S. and Modrich, P. (1979). J. Biol. Chem. 254, 11605-11614.
8. Bebenek, K. and Kunkel, T.A. (1989). Nucl. Acids Res.. 17, 5408.
9. Mattila, P., et al. (1991). Nucleic Acids Res.. 19, 4967-4973.

• Activity of DNA Modifying Enzymes in rCutSmart™ Buffer

• DNA Polymerase Selection Chart

1. Can T7 DNA Polymerase be used in other NEBuffers, including rCutSmart?
2. Can T7 DNA Polymerase be used to blunt DNA?
3. Can T7 DNA Polymerase be used to fill in 3' overhangs?
4. Can T7 DNA Polymerase be used to remove 5' overhangs?
5. Can T7 DNA Polymerase be heat inactivated?
6. Are deoxynucleotides needed to remove a 3' overhang using T7 DNA Polymerase?
7. When should native T7 DNA Polymerase be used?
8. What is the oligonucleotide-directed mutagenesis without phenotypic selection method?
9. Can the native T7 DNA Polymerase be used for sequencing?
10. Does NEB carry modified T7 Polymerase?
11. Can T7 DNA Polymerase be used in labeling reactions and partial fill in reactions?
12. Are NEB DNA Polymerases supplied with dNTPs?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0274S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0274S_L_v1_0111508
• M0274S_L_v1_0111603
• M0274S_L_v1_0111609
• M0274S_L_v1_0111703
• M0274S_L_v1_0111803
• M0274L_v1_10012703
• M0274S_v1_10021765
• M0274L_v1_10021767
• M0274L_v1_10039918
• M0274S_v1_10040917
• M0274L_v1_10057374
• M0274S_v1_10059218
• M0274L_v1_10075649
• M0274L_v1_10081788
• M0274S_v1_10091672
• M0274L_v1_10091094
• M0274S_v1_10107595
• M0274S_v1_10114913
• M0274S_v1_10123097
• M0274L_v1_10131164
• M0274S_v1_10135910
• M0274L_v1_10139934
• M0274L_v1_10152153
• M0274S_v1_10161568
• M0274L_v1_10161623
• M0274S_v1_10163746
• M0274S_v1_10165610
• M0274L_v1_10167009
• M0274L_v1_10174179
• M0274S_v1_10179183
• M0274L_v1_10180393

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• T7 DNA Polymerase (unmodified)
• T7 DNA Polymerase Reaction Buffer
• Recombinant Albumin, Molecular Biology Grade

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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Trademarks

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