Uracil-DNA Glycosylase (UDG)

Reagents Supplied

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0280S     -20       Uracil-DNA Glycosylase (UDG) M0280SVIAL -20 1 x 0.2 ml 5,000 units/ml   UDG Reaction Buffer B0280SVIAL -20 1 x 1.5 ml 10 X
M0280L     -20       Uracil-DNA Glycosylase (UDG) M0280LVIAL -20 1 x 1 ml 5,000 units/ml   UDG Reaction Buffer B0280SVIAL -20 1 x 1.5 ml 10 X

Application Features

• Treatment of 0.1 µg of uracil-containing DNA with 1 unit of UDG for 10 minutes at 37°C renders the DNA incapable of being copied by DNA polymerase. The enzyme can be 95% heat killed by incubation at 95°C for 10 minutes. Since UDG remains partially active following heat treatment at 95°C, it is recommended that uracil glycosylase inhibitor be added to prevent degradation of product DNA. Alternatively, reaction products can be immediately extracted with phenol/chloroform.

Companion Products

• Uracil Glycosylase Inhibitor (UGI)

Materials Sold Separately

• UDG Reaction Buffer

1. UDG is active over a broad pH range with an optimum at pH 8.0, does not require divalent cation, and is inhibited by high ionic strength (> 200 mM).

1. Lindahl, T. et al. (1977). J. Biol. Chem.. 252, 3286-3294.
2. Wang, Z. et al. (1991). Gene. 99, 31-37.
3. Devchand, P.R. et al. (1993). Nucl. Acids Res.. 21, 3437-3443.

• DNA Damage and PreCR

• Activities of DNA Repair Enzymes and Structure-specific Endonucleases
• DNA Repair Enzymes on Damaged and Non-standard Bases
• Properties of DNA Repair Enzymes and Structure-specific Endonucleases

1. What is the activity of UDG in the NEBuffers 1-4?
2. Will UDG work in T4 DNA ligase buffer?
3. What is the molecular weight of UDG?
4. Is UDG a tagged protein?
5. I see that UDG works optimally at 37°C. Do you have another glycosylase that works at higher temperatures?
6. Does the Uracil Glycosylase inhibitor (UGI) inhibit UDG?
7. Does UDG cut RNA?
8. Can UDG be used to remove dU from a short 21mer oligo? Do the dU residues need to be spaced in any special way to avoid problems with cleavage?
9. Are there any specific recommendations for the use of UDG on single stranded DNA? The materials on the web and data card seem to describe conditions for use with dsDNA.
10. What is the difference between UDG and UNG?
11. Does UDG release uracil from ss and dsDNA?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0280S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0280L_v1_10012361
• M0280S_v1_10012363
• M0280L_v1_10022676
• M0280S_v1_10022678
• M0280L_v1_10040497
• M0280L_v1_10049125
• M0280S_v1_10049126
• M0280L_v1_10050535
• M0280S_v1_10056012
• M0280L_v1_10057959
• M0280S_v1_10061554
• M0280L_v1_10061240
• M0280S_v1_10064359
• M0280L_v1_10077567
• M0280S_v1_10081987
• M0280L_v1_10087636
• M0280S_v1_10092226
• M0280L_v1_10097696
• M0280S_v1_10100971
• M0280L_v1_10108854
• M0280L_v1_10114622
• M0280L_v1_10124167
• M0280S_v1_10129602
• M0280L_v1_10130004
• M0280S_v1_10137303
• M0280S_v1_10151479
• M0280L_v1_10151476
• M0280S_v1_10173911

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Uracil-DNA Glycosylase (UDG)
• UDG Reaction Buffer

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.